• July 18, 2019 at 6:06 am #75801
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    A 2x supermix is now available containing either HF buffer or GC buffer, dNTPs, and Phusion polymerase. See references section below for links. Thermocycling conditions. 15-30 s/kb extension time. 98C for denaturation. Anneal at 3C above the lowest Tm if the primers are longer than 20nt, else at the Tm.
    In order to prevent these non-specific products, we can use hot-start polymerase. There are techniques to create hot-start enzymes, such as manual or physical separation and antibodies, or chemical modification. In the manual technique, one of the components of reaction mixture, e.g. Mg 2+ is added to the tube after the temperature exceeds 70
    Phusion High-Fidelity PCR Master Mix with HF Buffer is a 2X master mix consisting of Phusion DNA Polymerase, data card or product manual. Phusion ® DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. This product is manufactured by New England Biolabs
    Pork specific polymerase chain reaction assay was developed for authentication of meat and meat products. The primer pair was designed based on the D-loop region of mitochondrial gene.
    to Phusion Hot Start II DNA Polymerase reactions. Due to the novel nature of Phusion Hot Start II DNA Polymerase, the optimal reaction conditions may differ from PCR protocols for standard DNA polymerases. Due to the high salt concentration in the reaction Thermo Scientific Phusion Hot Start II High-Fidelity DNA Polymerase Product Information
    The Phusion High-Fidelity PCR Kit (large) contains a sufficient supply of Phusion High-Fidelity DNA Polymerase, Phusion HF and GC Buffers, deoxynucleotides, magnesium chloride, DMSO, and DNA size standard to perform 200 reactions. Control template and primers are provided for 20 control reactions.
    Phusion® Technology. Phusion DNA Polymerase brings together a novel Pyrococcus-like enzyme with a processivity-enhancing domain and generates PCR products with accuracy and speed previously unattainable with a single enzyme, even on your most difficult templates. Additionally, Phusion DNA Polymerase is capable of amplifying long templates.
    Phusion High-Fidelity DNA Polymerase is supplied with 5x Phusion HF Buffer, 5x Phusion GC Buffer, DMSO and 50 mM MgCl2 solution. Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1x concentration. 100U (2U/µL) Each for $111.20
    High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification.PCR reagents are kits that include researcher-supplied components, such as purified nucleic acid templates and oligonucleotide primers, along with components available through VWR, such as deoxyribonucleotides (dNTPs), and DNA polymerase.
    This is the PCR protocol for Phusion® High-Fidelity DNA Polymerase (M0530) This is the PCR protocol for Phusion® High-Fidelity DNA Polymerase (M0530)
    Instruction Manual Gibson Assembly ™ Master Mix. NEB #E2611S/L . 10/50 reactions. Phusion ® DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. Phusion DNA Polymerase is manufactured by New England Biolabs, Inc. under agreement with, and under the performance Phusion Hot Start II High Fidelity DNA Polymerase is the most accurate hot start DNA polymerase on the market. It combines the Phusion DNA Polymerase and a reversibly bound, specific Affibody ligand. Affibody inhibits the DNA polymerase activity at room temperature and thus prevents the amplification of nonspecific products.
    Instruction Manual Gibson Assembly ™ Master Mix. NEB #E2611S/L . 10/50 reactions. Phusion ® DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. Phusion DNA Polymerase is manufactured by New England Biolabs, Inc. under agreement with, and under the performance Phusion Hot Start II High Fidelity DNA Polymerase is the most accurate hot start DNA polymerase on the market. It combines the Phusion DNA Polymerase and a reversibly bound, specific Affibody ligand. Affibody inhibits the DNA polymerase activity at room temperature and thus prevents the amplification of nonspecific products.
    Phusion Hot Start Flex 2X Master Mix offers robust high fidelity performance and room temperature reaction setup in a convenient master mix format. The addition of an aptamer-based inhibitor allows room temperature reaction setup. The convenient master mix formulation is supplied at a 2X concentration and contains dNTPs and Mg++, requiring only the addition of primers and DNA template for
    PDF | In this study, a capillary polymerase chain reaction (cPCR) was applied for Salmonella detection from poultry meat. Salmonella detection limits of the optimized cPCR were determined with DNA

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